9 BULAN YANG MENAKJUBKAN PDF

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Menurut pandangan bank penggunaan internet banking membutuhkan biaya. Sulfolobus spp. In hyperthermophiles, B-family polymerase can detect uracil in DNA and stalls.

How it repairs or recruits repair machinery is not known Gorgan, Since complete NER and MMR systems are present in thermophilic Eubacteria, it appears that hyprethermophilic Archaea are different from thermophilic Eubacteria and have alternative strategies for excision repair of DNA.

In addition to these factors, photolyase gene activity amongst Archaea has been reported only in Sulfolobus solfataricu, Methanobacterium thermoautotrophicum Gorgan, and Picrophilus torridus Futterer et al.

This gene does not share similarity with any other microbe Skorvaga et al. Since photolyase appears to be uncommon amongst Archaea, it cannot be considered as a possible universal means of DNA repair in thermophile and hyperthermophilic Archaea but may be universal in thermophilic Eubacteria.

However, photolyase mediated transleasion repair mechanism in thermophilic Archaea remains illusive. It is believed that the thermophiles make efficient usage of genetic material by having multiple activities in one enzyme Sandigursky and Franklin ; Belova et al. High number of the exonuclease family and the accumulation of PIN-domain are reported in thermophilic Archaea and Eubacteria.

This augmentation is believed to help in DNA and RNA repair, which is required more due to damages caused at elevated temperature.

It is suggested that increase in PIN doamains in thermophile prokaryotes is to compensate and substitute lack of repairs systems in them. However, reasons for increase in number of exonuclease in mesophiles remain illusive Arcus, et al. Recombination repair systems are also reported to protect DNA at elevated temperatures as recA homologous to radA have been found to repair double stranded breaks in Pyrococcus furiosus even at 95C Di Ruggiero, , White, ; Gorgan, , Additionally it is also possible that some of the repair mechanisms are not unique in thermophiles.

However, significant difference is found in thermal stability where T. Therefore, it appears that it is the thermal stability of repair systems that may be important at least in thermophilic Eubacteria in addition to unique repair systems Gorgan, Many homologues of Archaeal repair systems in eubacteria and eukaryotes have been found see Aravind et al.

We agree with the proposal by Gorgan that possibly hyperthermophiles do not need efficient repair systems at the cost of vital cellular functions. The author also suggests that mutations due to replication or inefficient repair system may be beneficial to these organisms living under stressful conditions. RNA In order to survive the high temperature conditions, thermophiles have adopted strategies at the level of transcription too.

Investigations suggest that high CG content and external factors like high temperature may influence the usage of codons in RNA of thermophiles Lobry and Chessel, ; Singer and Hickey, Generally thermophiles have purine rich genome and it is speculated that purine load is preferred probably for efficient transcription of m-RNA and to avoid unnecessary double strand interaction.

Comparison between thermophiles and hyperthermophiles does not show significant differences in purine richness except on excluding Aeropyrum pernix. This may be due to high CG ratio in this organism Paz et al. However, in hyperthermophilic Pyrobaculum aerophilum long tracts of purines have been found in coding and noncoding regions.

Hence, it is suggested that purine richness could not be of significant adaptive value, as it is not preferably present in coding regions alone Fitz-Gibbon et al.

This preference is in order to avoid undesirable bonds with other RNA and specially to avoid any 66 Seema Trivedi et al. Purine richness may be helpful in high transcription rate of many mRNA from pyrimidine rich template and may be more stable to spontaneous hydrolysis at high temperature Paz et al.

9 bulan yang menakjubkan pdf

It is also evident that besides nucleotide content of a genome, the second most important factor is the optimal growth temperature, which decides the preference for codon usage.

It has been found that thermophiles have a different preference for synonymous codon usage from that of mesophiles Lobry and Chessel e. This must be primarily for not only ensuring stable pre-mRNA transcript and mRNA but also for translation at high temperatures. But additionally it appears that tRNA abundance may also play an important role in preferential codon usage. Possibly some elusive selective forces at higher temperatures promote this kind of nucleotide content in genome Lynn et al.

Thermophiles are expected to avoid of 5-UpA3 and 5-CpA-3 phosphodiester bonds in RNAs but this has not been observed in thermophilic prokaryotes. Therefore, it appears that selective pressures have not acted against these bonds even though they are susceptible to hydrolysis.

It is possible that these thermophilic prokaryotes either do not need to avoid these bonds or there may be other mechanism that prevents hydrolysis or stabilises RNA Lobry and Chessel, Survival strategies extend to provide thermal stability to t-RNA and in particular to aminoacyl tRNA which is labile at high temperatures but not at lower temperatures Stepanov and Nyborg, , and possibly several factors help in stabilizing RNA in thermophilic Eubacteria.

These factors are high CG content, short length of RNA, increase in hydrogen bonds in helices, minimizing alternative folding, additional Watson-Crick base pairs at the base of stem loop and shortened connections between helices Brown et al. These factors may be important in thermophilic Archaea also. However, it has been observed that higher content of dihydrouridin is present in thermostable t-RNA of other thermophiles but is underrepresented in Archaea. Therefore, at least in most thermophilic Archaea, dihydrouridin may not be important for t-RNA stability at high temperature.

In fact, its absence is considered to provide thermostability in Archaea. It has also been reported that post-transcription modified nucleotides e. This type of post-transcriptional modification may be universally employed by thermophilic Archaea and Eubacteria.

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Besides this, archaeosine, a derivative of 7-deazaguanosine is exclusively present in the D-loop of Archaea t-RNA Watanabe et al. In addition to these factors, high number of ribose methylated nucleotides and presence of Mg ions, help in stabilization of t-RNA Edmonds et al. Reports on contribution of high CG content being important for thermal stability is contradicted by the finding that report no significant differences in the CG content in t-RNA of cold-adapted Archaea and thermophiles.

Therefore, it appears that additional factors provide thermal stability to t-RNA at high temperature Saunders et al. It is also possible that rigidity rendered to t-RNA in thermophiles provides protection again thermal damages and hence in some cases there are no differences in CG contents of thermophiles and psychrophiles.

In the studies carried out so far, since only few thermophile Eubacterial genomes have been included, it is not possible at present to deduce whether all thermophilic Eubacteria have adaptations that are similar to Archaea. Conclusion From the forgoing information it is apparent that thermophilic Archaea and Eubacteria have adapted to high temperature conditions or possibly because of their unique features they have been able to survive in these conditions.

Whether evolutionary forces induced changes in DNA that are reflected in expression or whether it was the Nucleid acid stability 67 necessity of undergoing modification at the expression level that the DNA had to undergo changes still remains debatable. Differences in repair systems between the two domains also suggest that both NER and MMR systems are either absent or have not been detected in hyperthermophilic Archaea. We agree with Gorgan suggesting a possibility of evolutionarily and functionally divergent repair pathways in hyperthermophilic Archaea that do not show similarity with well known established systems.

So far only few thermophilic Eubacteria have been chosen for comparison with thermophilc Archcaea which have revealed few differences between the two domains regarding the strategies used for thermoprotection.

The situation is compounded by the fact that the two thermophilc Eubacteria used for studies, are considered close to Archaea in lineage and appear to share many genes with Archaea possibly due to horizontal gene transfer. In future, understanding and discoveries in more thermophilic bacteria if more exist would probably help in comparing the strategies of the two domains.

These differences and similarities may help in understanding the specific strategies that are common and necessary for surviving at high temperature. Conserved domains in DNA repair proteins and evolution of repair systems. Nucleic Acids Res. Distant structural homology leads to the functional characterization of an archaeal PIN domain as an exonuclease.

J Biol Chem. Both DNA and histone fold sequences contribute to archaeal nucleosome stability. A complete sequence of the T. Genome Res. Proc Natl Acad Sci. USA , , Characterization of ribonuclease P RNAs from thermophilic bacteria.

Bujinicki JM and Radlinsks M. Molecular evolution of DNA- cytosine-N4 methyltransferases: evidence for their polyphyletic origin. Repair of extensive ionizing-radiation DNA damage at 95 degrees C in the hyperthermophilic archaeon Pyrococcus furiosus.

J Bacteriol. Doolittle RF. Of Archaea and Eo: What's in a name? DNA methylation in thermophilic bacteria: N4-methylcytosine, 5methylcytosine, and N6-methyladenine. Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum aerophilum.

Genome sequence of Picrophilus torridus and its implications for life around pH 0. A nonhyperthermophilic common ancestor to extant life forms. Science, , Gorgan DW. Hyperthermophiles and the problem of DNA instability.

Molec Microbiol. The question of DNA repair in hyperthermophilic Archaea. Trends in Microbiology, 8: , Issues Mol. Adaptive role of increased frequency of polypurine tracts in mRNA sequences of thermophilic prokaryotes. Extreme resistance to thermally induced DNA backbone breaks in the hyperthermophilic archaeon Pyrococcus furiosus.

Sandigursky M and Franklin WA. Curr Biol. Growth-phase-dependent synthesis of histones in the archaeon Methanothermus fervidus. Mechanisms of thermal adaptation revealed from the genomes of the Antarctic Archaea Methanogenium frigidum and Methanococcoides burtonii.

Thermophilic prokaryotes have characteristic patterns of codon usage, amino acid composition and nucleotide content.

Gene, , Thermostable archaeal O6-alkylguanine-DNA alkyltransferases. Stepanov VG and Nyborg J. Thermal stability of aminoacyltRNAs in aqueous solutions. Extremophiles, 6: , Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to Archaeal tRNA, proceeds via a pathway involving base replacement on the trna polynucleotide chain.The situation is compounded by the fact that the two thermophilc Eubacteria used for studies, are considered close to Archaea in lineage and appear to share many genes with Archaea possibly due to horizontal gene transfer.

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Dengan menghubungkan Adobe Reader ke Anda Dropbox akun atau akun Anda juga dapat menelusuri dan membuka file berbasis cloud Anda. I agree. This may be due to high CG ratio in this organism Paz et al. For detailed program information, More information. Kami menemukan sukacita yang baru untuk membagikan kebaikan Tuhan. Analisis Gabungan 7 2 Penyokong akan memberi nasihat. Sandigursky M and Franklin WA.